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Lookup NU author(s): Dr Mark Banfield
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The protein PAE2307 is a member of a protein family of unknown function which is conserved among a number of bacterial and archaeal species. The protein was overexpressed in Escherichia coli, purified and crystallized in two crystal forms. The prevalent form was twinned, but the other diffracted to 1.45 Å resolution. The non-twinned crystals proved difficult to reproduce, so screening of potential heavy-atom derivatives by native polyacrylamide gel electrophoresis was used to establish suitable derivatization conditions. This process enabled the production of a K2Pt(NO2)4 derivative that was used to collect a single-wavelength anomalous diffraction (SAD) data set from the only available crystal. Phase information of high quality was obtained, enabling the calculation of an interpretable electron-density map.
Author(s): Banfield MJ; Lott JS; Sigrell J; Baker EN
Publication type: Article
Publication status: Published
Journal: Acta Crystallographica D: Biological Crystallography
ISSN (print): 0907-4449
Publisher: Wiley-Blackwell Publishing, Inc.
PubMed id: 14646083
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