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Hypertonic activation of phospholemman in solitary rat hepatocytes in primary culture

Lookup NU author(s): Dr Andreas Werner


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Under hypertonic conditions, solitary rat hepatocytes in primary culture shrink and subsequently exhibit a distinct regulatory volume increase (RVI). Reverse-transcribed polymerase chain reaction and 5′ and 3′ RACE (rapid amplification of cDNA ends) techniques reveal that these cells express phospholemman (PLM). In whole-cell recordings, the hypertonic activation of a channel is observed that resembles PLM with respect to unitary conductance (600-700 pS), gating pattern, and non-selectivity for Na+ over K+. In Xenopus oocytes expressing hepatocyte PLM, hypertonic stress induces a non-selective cation conductance and noise analysis reveals the activation of a channel with a unitary conductance of approximately 700 pS. These results suggest a role of PLM in the RVI of rat hepatocytes. © 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Publication metadata

Author(s): Kirschner U, Van Driessche W, Werner A, Wehner F

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2003

Volume: 537

Issue: 1-3

Pages: 151-156

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier BV


DOI: 10.1016/S0014-5793(03)00115-7

PubMed id: 12606048


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