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Lookup NU author(s): Dr Andreas Werner
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Under hypertonic conditions, solitary rat hepatocytes in primary culture shrink and subsequently exhibit a distinct regulatory volume increase (RVI). Reverse-transcribed polymerase chain reaction and 5′ and 3′ RACE (rapid amplification of cDNA ends) techniques reveal that these cells express phospholemman (PLM). In whole-cell recordings, the hypertonic activation of a channel is observed that resembles PLM with respect to unitary conductance (600-700 pS), gating pattern, and non-selectivity for Na+ over K+. In Xenopus oocytes expressing hepatocyte PLM, hypertonic stress induces a non-selective cation conductance and noise analysis reveals the activation of a channel with a unitary conductance of approximately 700 pS. These results suggest a role of PLM in the RVI of rat hepatocytes. © 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Author(s): Kirschner U, Van Driessche W, Werner A, Wehner F
Publication type: Article
Publication status: Published
Journal: FEBS Letters
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
Publisher: Elsevier BV
PubMed id: 12606048
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