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Effect of starvation on proteases in insecticide-resistant and susceptible strains of Musca domestica

Lookup NU author(s): Professor Mushtaq Saleem, Dr Richard Wilkins, Dr David Mantle

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Abstract

To determine the possible involvement of the process of intracellular protein catabolism in the development of insecticide resistance, we have compared the activities of a comprehensive range of cytoplasmic and lysosomal proteolytic enzymes in 48 hours starved and un-starved resistant and susceptible strains of Musca domestica. Compared to un-starved, the homogenates of 48 hours starved house flies of both resistant and susceptible strains showed elevated levels of most of the poteases tested in this study. Thus in susceptible strain, leucyl aminopeptidase, proline endopeptidase and cathepsin L showed elevated levels in starved than un-starved by 42%, 28% and 23%, respectively, while the remaining proteases exhibited minor fluctuations. On the other hand, in resistant strain, leucyl aminopeptidase (58%), proline endopeptidase (41%) and cathepsin L (38%) showed elevated levels whereas the remaining proteases exhibited minor deviations. The results suggested that higher induction of proteases in starved resistant than susceptible strain may be important in the development of insecticide resistance in M. domestica.


Publication metadata

Author(s): Saleem MA, Wilkins RM, Mantle D, Ahmad S, Shakoori AR

Publication type: Article

Publication status: Published

Journal: Pakistan Journal of Zoology

Year: 2003

Volume: 35

Issue: 4

Pages: 311-318

Print publication date: 01/01/2003

ISSN (print): 0030-9923

ISSN (electronic):

Publisher: Zoological Society of Pakistan


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