Browse by author
Lookup NU author(s): Professor Andrew Whiteley,
Professor Anthony O'Donnell
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0-5-, 5-10- and 10-15-cm samples represented organic horizons whilst the 15-20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5-10-cm and 10-15-cm organic horizons for the July samplings whilst the 15-20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Author(s): Griffiths RI, Whiteley AS, O'Donnell AG, Bailey MJ
Publication type: Article
Publication status: Published
Journal: FEMS Microbiology Ecology
ISSN (print): 0168-6496
ISSN (electronic): 1574-6941
Publisher: Wiley-Blackwell Publishing
PubMed id: 19719694
Altmetrics provided by Altmetric