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Uracil recognition by archaeal family B DNA polymerases

Lookup NU author(s): Professor Bernard Connolly, Dr Brian Wilson

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Abstract

Archaeal family-B DNA polymerases possess a novel uracil-sensing mechanism. A specialized pocket scans the template, ahead of the replication fork, for the presence of uracil; on encountering this base, DNA synthesis is stalled. The structural basis for uracil recognition by polymerases is described and compared with other uracil-recognizing enzymes (uridine-triphosphate pyrophophatases and uracil-DNA glycosylases). Remarkably, protein-protein interactions between all three archaeal uracil sensors are observed; possibly the enzymes co-operate to efficiently eliminate uracil from archaeal genomes.

Publication metadata

Author(s): Connolly BA, Fogg MJ, Shuttleworth G, Wilson BT

Publication type: Article

Publication status: Published

Journal: Biochemical Society Transactions

Year: 2003

Volume: 31

Issue: 3

Pages: 699-702

ISSN (print): 0300-5127

ISSN (electronic): 1470-8752

Publisher: Portland Press Ltd.

URL: http://dx.doi.org/10.1042/BST0310699

DOI: 10.1042/BST0310699

PubMed id: 12773186

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