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Lookup NU author(s): Dr Alexei von Delwig,
Professor John Robinson
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The subcellular localization of peptide/MHC complexes was investigated during processing of the surface M5 protein from Streptococcus pyogenes. Bone marrow-derived macrophages were pulsed with viable S. pyogenes for 20 min followed by various periods of chase. T hybridoma cells detected complexes of one epitope, M517-31 with Ed on the surface of macrophages within 30 min of chase. In contrast, complexes with another epitope, M5308-319 with Ad peaked later. Intracellular localization of peptide/MHC-II complexes was studied by subcellular fractionation and detection of complexes in fractions by T hybridoma cells. M517-31/Ed complexes were detected in light membrane fractions containing plasma membrane and early endosomes by 10-30 min. M5308-319/Ad complexes were detected in these light membranes after 3 h of chase. Thus, the time course of M5308-319/Ad presentation was delayed relative to M517-31/Ed. However, neither type of complex was detected at any time in fractions containing phagosomes. Both species of peptide/MHC complexes localized to endocytic compartments, indicating a role for endosomes in presentation of antigens from phagocytosed bacteria.
Author(s): von Delwig A, Ramachandra L, Harding CV, Robinson JH
Publication type: Article
Publication status: Published
Journal: European Journal of Immunology
ISSN (print): 0014-2980
ISSN (electronic): 1521-4141
Publisher: Wiley - VCH Verlag GmbH & Co. KGaA
PubMed id: 12938211
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