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Synergistic and antagonistic interactions of anticholinesterase terpenoids in Salvia lavandulaefolia essential oil

Lookup NU author(s): Dr Sergey Savelev, Dr Edward Okello, Dr Richard Wilkins, Emeritus Professor Elaine Perry

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Abstract

In vitro anticholinesterase activities of eight commercially available terpenoid constituents of Salvia lavandulaefolia have been investigated. These included 1,8-cineole, camphor, α-pinene, β-pinene, borneol, caryophyllene oxide, linalool and bornyl acetate. Dose-dependent inhibition of acetylcholinesterase (AChE) by these chemical constituents was determined using the method of Ellman [Biochem. Pharmacol. 7 (1961) 88]. The IC50 value of 1,8-cineole was 0.06±0.01 mg/ml similar to that of the essential oil (0.05±0.01 mg/ml). Analyses of the expected inhibitions based on the prediction of a zero interactive response of a combination at its naturally occurring ratios were carried out in comparison with observed inhibition. Minor synergy was apparent in 1,8-cineole/α-pinene and 1,8-cineole/caryophyllene oxide combinations, with interaction indexes not exceeding 0.5. In contrast, a combination of camphor and 1,8-cineole was antagonistic with an interaction index of 2. A combination of all eight compounds was zero interactive. A combination of six constituents, excluding 1,8-cineole and camphor, was used to compare the method of expected response of a combination with a method of summation. These findings reveal that the inhibitory activity of the oil results from a complex interaction between its constituents, which produce both synergistic and antagonistic responses between the component terpenes. Understanding such interactions is important in comparing species on the basis of chemical composition. © 2003 Elsevier Science Inc. All rights reserved.


Publication metadata

Author(s): Savelev S, Okello E, Perry NSL, Wilkins RM, Perry EK

Publication type: Article

Publication status: Published

Journal: Pharmacology Biochemistry and Behavior

Year: 2003

Volume: 75

Issue: 3

Pages: 661-668

Print publication date: 01/06/2003

ISSN (print): 0091-3057

ISSN (electronic): 1873-5177

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/S0091-3057(03)00125-4

DOI: 10.1016/S0091-3057(03)00125-4

PubMed id: 12895684


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