Browse by author
Lookup NU author(s): Dr Sergey Savelev,
Dr Edward Okello,
Dr Richard Wilkins,
Emeritus Professor Elaine Perry
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
In vitro anticholinesterase activities of eight commercially available terpenoid constituents of Salvia lavandulaefolia have been investigated. These included 1,8-cineole, camphor, α-pinene, β-pinene, borneol, caryophyllene oxide, linalool and bornyl acetate. Dose-dependent inhibition of acetylcholinesterase (AChE) by these chemical constituents was determined using the method of Ellman [Biochem. Pharmacol. 7 (1961) 88]. The IC50 value of 1,8-cineole was 0.06±0.01 mg/ml similar to that of the essential oil (0.05±0.01 mg/ml). Analyses of the expected inhibitions based on the prediction of a zero interactive response of a combination at its naturally occurring ratios were carried out in comparison with observed inhibition. Minor synergy was apparent in 1,8-cineole/α-pinene and 1,8-cineole/caryophyllene oxide combinations, with interaction indexes not exceeding 0.5. In contrast, a combination of camphor and 1,8-cineole was antagonistic with an interaction index of 2. A combination of all eight compounds was zero interactive. A combination of six constituents, excluding 1,8-cineole and camphor, was used to compare the method of expected response of a combination with a method of summation. These findings reveal that the inhibitory activity of the oil results from a complex interaction between its constituents, which produce both synergistic and antagonistic responses between the component terpenes. Understanding such interactions is important in comparing species on the basis of chemical composition. © 2003 Elsevier Science Inc. All rights reserved.
Author(s): Savelev S, Okello E, Perry NSL, Wilkins RM, Perry EK
Publication type: Article
Publication status: Published
Journal: Pharmacology Biochemistry and Behavior
Print publication date: 01/06/2003
ISSN (print): 0091-3057
ISSN (electronic): 1873-5177
PubMed id: 12895684
Altmetrics provided by Altmetric