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Interleukin 4 production by peripheral blood lymphocytes in patients with classical Hodgkin lymphoma

Lookup NU author(s): Dr Tryfonia Mainou-Fowler, Professor Stephen Proctor, Dr Penelope Taylor


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Although the production of interleukin (IL) 2 and interferon (IFN) γ by peripheral blood lymphocytes in patients with Hodgkin lymphoma (HL) is well documented, the synthesis of IL4 has not been investigated before. The present study examines the production of IL4 by 2-day phytohaemaglutinin (PHA)-stimulated peripheral blood (PB) cells in HL and correlates the cytokine levels with the proportion of the different T-cell sub-populations. We observed a significant increase in the mean level of production of IL4 in patients with HL when compared with normal controls. The increased amount of IL4 in patients with HL correlated significantly with the proportion of the CD3 +CD8+ cells but not with CD3+CD4+. The intensity of cytoplasmic IL4 (expressed as relative median fluorescence (RMF)) was significantly higher in the CD3+CD8+ cells of the patients with HL compared with the CD3+CD4+ sub-population, or with the normal CD3+CD8+ cells and correlated with the levels of IL4 release in culture supernatants. In conclusion, there is increased production of IL4 by PHA-activated PB lymphocytes in HL. The CD3+CD8+ T-cell population appears to be responsible for this increased synthesis. © 2003 Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Mainou-Fowler, T., Proctor, S. J., Taylor, P. R. A.

Publication type: Article

Publication status: Published

Journal: Leukemia Research

Year: 2004

Volume: 28

Issue: 2

Pages: 159-166

Print publication date: 01/02/2004

ISSN (print): 0145-2126

ISSN (electronic): 1873-5835


DOI: 10.1016/S0145-2126(03)00216-9

PubMed id: 14654080


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