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Phylogenetic placement of Botryococcus braunii (Trebouxiophyceae) and Botryococcus sudeticus isolate UTEX 2629 (Chlorophyceae)

Lookup NU author(s): Hoda Senousy, Dr Gordon Beakes, Dr Ethan Hack

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Abstract

The phylogenetic placement of four isolates of Botryococcus braunii Ku╠łtzing and of Botryococcus sudeticus Lemmermann isolate UTEX 2629 was investigated using sequences of the nuclear small subunit (18S) rRNA gene. The B. braunii isolates represent the A (two isolates), B, and L chemical races. One isolate of B. braunii (CCAP 807/1; A race) has a group I intron at Escherichia coli position 1046 and isolate UTEX 2629 has group I introns at E. coli positions 516 and 1512. The rRNA sequences were aligned with 53 previously reported rRNA sequences from members of the Chlorophyta, including one reported for B. braunii (Berkeley strain). Phylogenetic trees were constructed using distance, weighted maximum parsimony, and maximum likelihood, and their reliability was estimated using bootstrap analysis for distance and parsimony and Bayesian inference for likelihood. All methods showed, with high bootstrap or credibility support, that the four isolates of B. braunii form a monophyletic group whose closest relatives are in the genus Choricystis in the Trebouxiophyceae, whereas the previously reported B. braunii sequence is from a member of the Chlamydomonadales in the Chlorophyceae and isolate UTEX 2629 is a member of the Sphaeropleales in the Chlorophyceae. Polyphyly of these sequences was confirmed by Kishino-Hasegawa tests on artificial trees in which sequences were moved to a single lineage.


Publication metadata

Author(s): Senousy HH, Beakes GW, Hack E

Publication type: Article

Publication status: Published

Journal: Journal of Phycology

Year: 2004

Volume: 40

Issue: 2

Pages: 412-423

Print publication date: 01/04/2004

ISSN (print): 0022-3646

ISSN (electronic): 1529-8817

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1046/j.1529-8817.2004.03173.x

DOI: 10.1046/j.1529-8817.2004.03173.x


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