Toggle Main Menu Toggle Search

Open Access padlockePrints

A novel copper site in a cyanobacterial metallochaperone

Lookup NU author(s): Dr Gilles Borrelly, Dr Clive Butler, Professor Nigel Robinson


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


The thylakoid lumen of the cyanobacterium Synechocystis PCC 6803 is supplied with copper via two copper-transporting ATPases anc a metallochaperone intermediary. We show that the copper site of this metallochaperone is unusual and consists of two cysteine residues and a histidine imidazole located on structurally dynamic loops. Substitution of this histidine residue enhances bacterial two-hybrid interaction with the cytosolic copper exporter, but not the copper importer, suggesting that the interacting surfaces are distinct, with implications for metal transfer.

Publication metadata

Author(s): Borrelly GPM, Blindauer CA, Schmid R, Butler CS, Cooper CE, Harvey I, Sadler PJ, Robinson NJ

Publication type: Article

Publication status: Published

Journal: Biochemical Journal

Year: 2004

Volume: 378

Issue: 2

Pages: 293-297

Print publication date: 01/03/2004

ISSN (print): 0264-6021

ISSN (electronic): 1470-8728

Publisher: The Biochemical Society


DOI: 10.1042/BJ20031669

PubMed id: 14711369


Altmetrics provided by Altmetric