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Lookup NU author(s): Professor Rick Lewis
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The process of spore formation in Bacillus subtilis is dependent upon a sophisticated program of gene expression that is regulated both temporally and spatially by a series of alternate sigma factors, in conjunction with a number of transcriptional regulators. One of these, SpoVT, regulates forespore-specific σG-dependent transcription and is related at the amino acid level to the major stationary phase sentinel, AbrB, whose mode of DNA recognition appears to be non-classical. Here, we report that the C-terminal domain of SpoVT is crucial to its correct folding and function, and how the DNA-binding domain from AbrB cannot complement the closely homologous domain of SpoVT in vivo. We also establish the oligomeric state of SpoVT and its component domains. Finally, we demonstrate that the regulation of transcriptional control by SpoVT is unexpectedly more complicated than its counterpart, AbrB, and that the latent non-specific DNA-binding activity of the N-terminal domain of SpoVT is modulated by the C-terminal domain, which perhaps in combination with another unknown factor, confers specificity. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Author(s): Dong TC, Cutting SM, Lewis RJ
Publication type: Article
Publication status: Published
Journal: FEMS Microbiology Letters
Year: 2004
Volume: 233
Issue: 2
Pages: 247-256
Print publication date: 15/04/2004
ISSN (print): 0378-1097
ISSN (electronic): 1574-6968
Publisher: Wiley-Blackwell Publishing Ltd.
URL: http://dx.doi.org/10.1016/j.femsle.2004.02.013
DOI: 10.1016/j.femsle.2004.02.013
PubMed id: 15063493
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