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Characterization of nine microsatellite loci in Cynoglossum officinale (Boraginaceae)

Lookup NU author(s): Dr Kirsten Wolff

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Abstract

Cynoglossum officinale is a biennial plant pollinated by bumblebees. We developed microsatelllite loci in order to study the population genetic structure and effects of inbreeding in this species. In this paper, we describe nine polymorphic microsatellites for C. officinale. Between two and four alleles per locus were observed in a sample of 20 individuals from one population. Multiplexing allowed the seven most useful loci to be genotyped using three polymerase chain reaction (PCR) reactions.

Publication metadata

Author(s): Korbecka G, Wolff K

Publication type: Article

Publication status: Published

Journal: Molecular Ecology Notes

Year: 2004

Volume: 4

Issue: 2

Pages: 229-230

Print publication date: 01/06/2004

ISSN (print): 1471-8278

ISSN (electronic): 1755-0998

Publisher: Wiley

URL: http://dx.doi.org/10.1111/j.1471-8286.2004.00625.x

DOI: 10.1111/j.1471-8286.2004.00625.x

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