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In vivo effects of cyfluthrin on proteolytic enzyme activities of malathion-resistant and susceptible strains of Tribolium castaneum

Lookup NU author(s): Professor Mushtaq Saleem, Dr Richard WilkinsORCiD, Dr David Mantle


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To elucidate whether insecticide toxicity in insects involves insecticide - induced abnormalities of the intracellular protein catabolic process, we have determined the in vivo effect of a synthetic pyrethroid, cyfluthrin on the activities of representative protein catabolising cytoplasmic and lysosomal proteases (responsible for the various stages of the protein degradation cascade and essential for normal cell functioning) in resistant and susceptible strains of Tribolium castaneum. Effect of cyfluthrin was determined at LC50 after 48 hour of treatment both in the live and dead adult beetles and compared with controls. In treated live beetles, cyfluthrin decreased alanyl aminopeptidase (18%); arginyl aminopeptidase (6%), leucyl aminopeptidase (22%), tripeptidyl aminopeptidase (12%), proline endopeptidase (43%) and dipeptidyl aminopeptidase I (23%) and increased cathepsin H (42%), while in dead beetles, almost all cytoplasmic proteases as well as cathepsin H manifested further decreasing trend. On the other hand in treated resistant strain live beetles, all lysosomal proteases and leucyl aminopeptidase were considerably decreased ranging from 19% to 58% of control activity and this decreasing trend was further intensified in treated dead beetles, which ranged from 30% to 96%. We conclude that the effect of cyfluthrin on proteolytic enzyme activities induced inhibition of proteases and could be important in the development of insecticide resistance in T. castaneum. Copyright 2004 Zoological Society of Pakistan.

Publication metadata

Author(s): Saleem MA, Wilkins RM, Mantle D, Shakoori AR

Publication type: Article

Publication status: Published

Journal: Pakistan Journal of Zoology

Year: 2004

Volume: 36

Issue: 3

Pages: 239-245

Print publication date: 01/01/2004

ISSN (print): 0030-9923

ISSN (electronic):

Publisher: Zoological Society of Pakistan