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Lookup NU author(s): Dr Nicholas Allenby, Nicola O'Connor, Dr Zoltan Pragai, Dr Noel Carter, Professor Anil Wipat, Emeritus Professor Alan Ward, Professor Colin Harwood
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During phosphate starvation, Bacillus subtilis regulates genes in the PhoP regulon to reduce the cell's requirement for this essential substrate and to facilitate the recovery of inorganic phosphate from organic sources such as teichoic and nucleic acids. Among the proteins that are highly induced under these conditions is PstS, the phosphate-binding lipoprotein component of a high-affinity ABC-type phosphate transporter. PstS is encoded by the first gene in the pst operon, the other four members of which encode the integral membrane and cytoplasmic components of the transporter. The transcription of the pst operon was analysed using a combination of methods, including transcriptional reporter gene technology, Northern blotting and DNA arrays. It is shown that the primary transcript of the pst operon is processed differentially to maintain higher concentrations of PstS relative to other components of the transporter. The comparative studies have revealed limitations in the use of reporter gene technology for analysing the transcription of operons in which the messenger RNA transcript is differentially processed. © 2004 SGM.
Author(s): Allenby NEE, O'Connor N, Pragai Z, Carter NM, Miethke M, Engelmann S, Hecker M, Wipat A, Ward AC, Harwood CR
Publication type: Article
Publication status: Published
Journal: Microbiology
Year: 2004
Volume: 150
Issue: 8
Pages: 2619-2628
Print publication date: 01/08/2004
ISSN (print): 1350-0872
ISSN (electronic): 1465-2080
Publisher: Society for General Microbiology
URL: http://dx.doi.org/10.1099/mic.0.27126-0
DOI: 10.1099/mic.0.27126-0
PubMed id: 15289558
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