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Microfiltration of whey proteins adsorbed on yeast cells

Lookup NU author(s): Dr Bulent Keskinler, Dr Elif Erhan, Professor Galip Akay


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Soluble whey proteins (WPs), adsorbed on yeast cells, were recovered by a crossflow microfiltration (MF) technique using a cellulose nitrate membrane with a pore size of 0.45 μm. The crossflow velocity was 1.5 ms-1 with a transmembrane pressure of 200 kPa at 25°C. A series of protein rejections occured at various pH values ranging from 2 to 8. WPs adsorbed more on to yeast cells at low pH (pH < 4) than at high pH values, probably because they were positively charged at low pH. It was also shown that permeate flux increased and Modified Membrane Fouling Index values decreased at low pH levels. When the yeast concentration was 50 g L-1, the flux decreased five times compared with that in the absence of yeast. Protein recovery increased with increasing yeast concentrations. The highest protein recovery was found to be 85% at a yeast concentration of 50 g L-1 at a steady state flux rate of 10-6 ms-1 at 25°C. When diluted solutions of whey were used, the same rejection of protein, adsorbed on yeast cells, was achieved at ten times lower amounts of yeast cells. This technique not only provides for the recovery of protein but also may give rise to the direct use of yeast cells, which are rich in protein, in the baking industry. WPs absorbed by yeast cells can be used to produce nutritionally rich products in areas where yeasts have been already used.

Publication metadata

Author(s): Keskinler B, Erhan E, Akay G, Kaya M, Bayguven B

Publication type: Article

Publication status: Published

Journal: International Journal of Food Science and Technology

Year: 2004

Volume: 39

Issue: 1

Pages: 71-78

ISSN (print): 0950-5423

ISSN (electronic): 1365-2621

Publisher: Wiley-Blackwell


DOI: 10.1046/j.0950-5423.2003.00756.x


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