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Putative involvement of the histone acetyltransferase Tip60 in ribosomal gene transcription

Lookup NU author(s): Dr Ian Logan, Susan Cook, Professor David Neal, Professor Craig Robson

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Abstract

Tip60 is a histone acetyltransferase (HAT) implicated in a wide range of cellular functions, including mRNA synthesis and DNA repair. In the present report we propose a model based on which Tip60 is actively involved in ribosomal gene transcription through acetylation of UBF, a ribosomal specific transcription factor, as well as through its direct recruitment to the human ribosomal gene promoter, as shown by chromatin immunoprecipitation experiments. Electron microscopy studies revealed that Tip60 resides in sites of active rDNA transcription within the nucleolus, while it co-localizes with UBF as shown by confocal microscopy. In addition, in vivo transcription assays demonstrated that the nucleolar fraction of Tip60 localizes to sites of newly synthesized rRNA. Finally, functional assays established that Tip60 complexes with, and targets UBF for acetylation. The present study underlines the importance of acetylation in rDNA transcription and directly implicates Tip60 in the process of ribosomal gene transcription. © Oxford University Press 2004; all rights reserved.

Publication metadata

Author(s): Halkidou, K., Logan, I. R., Cook, S., Neal, D. E., Robson, C. N.

Publication type: Article

Publication status: Published

Journal: Nucleic Acids Research

Year: 2004

Volume: 32

Issue: 5

Pages: 1654-1665

Print publication date: 01/03/2004

Date deposited: 08/02/2012

ISSN (print): 0305-1048

ISSN (electronic): 1362-4954

URL: http://dx.doi.org/10.1093/nar/gkh296

DOI: 10.1093/nar/gkh296

PubMed id: 15016909

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