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Differential efficacy of vaccinia virus envelope proteins administered by DNA immunisation in protection of BALB/c mice from a lethal intranasal poxvirus challenge

Lookup NU author(s): Dr Simon Bridge, Professor John Robinson

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Abstract

DNA vaccines might offer an alternative to the live smallpox vaccine in providing protective efficacy in an orthopoxvirus (OPV) lethal respiratory challenge model. BALB/c mice were immunised with DNA vaccines coding for 10 different single vaccinia virus (VACV) membrane proteins. After an intranasal challenge with the VACV IHD strain, three gene candidates B5R, A33R and A27L produced ≥66% survival. The B5R DNA vaccine consistently produced 100% protection and exhibited greatest efficacy after three 50 μg intramuscular doses in this model. Sero-conversion to these vaccines was often inconsistent, implying that antibody itself was not a correlate of protection. The B5R DNA vaccine induced a strong and consistent gamma interferon (IFNγ) response in BALB/c mice given a single DNA vaccine dose. Strong IFNγ responses were also measured in pTB5R immunised C57BL6 mice deficient for MHC class I molecules, suggesting that the memory response was mediated by a CD4+ T cell population. Crown Copyright © 2004 Published by Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Pulford DJ, Gates A, Bridge SH, Robinson JH, Ulaeto D

Publication type: Article

Publication status: Published

Journal: Vaccine

Year: 2004

Volume: 22

Issue: 25-26

Pages: 3358-3366

Print publication date: 03/09/2004

Date deposited: 16/10/2010

ISSN (print): 0264-410X

ISSN (electronic): 1873-2518

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.vaccine.2004.02.034

DOI: 10.1016/j.vaccine.2004.02.034

PubMed id: 15308360

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