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Lookup NU author(s): Christopher Blackwell,
Dr Amanda Greenall,
Dr Simon Whitehall
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HIRA-like (Hir) proteins are evolutionarily conserved and are implicated in the assembly of repressive chromatin. In Saccharomyces cerevisiae, Hir proteins contribute to the function of centromeres. However, S. cerevisiae has point centromeres that are structurally different from the complex centromeres of metazoans. In contrast, Schizosaccharomyces pombe has complex centromeres whose domain structure is conserved with that of human centromeres. Therefore, we examined the functions of the fission yeast Hir proteins Slm9 and the previously uncharacterised protein Hip1. Deletion of hip1+ resulted in phenotypes that were similar to those described previously for slm9Δ cells: a cell cycle delay, synthetic lethality with cdc25-22, and poor recovery from nitrogen starvation. However, while it has previously been shown that Slm9 is not required for the periodic expression of histone H2A, we found that loss of Hip1 led to derepression of core histone genes expression outside of S phase. Importantly, we found that deletion of either hip1+ or slm9 + resulted in increased rates of chromosome loss, increased sensitivity to spindle damage, and reduced transcriptional silencing in the outer centromeric repeats. Thus, S. pombe Hir proteins contribute to pericentromeric heterochromatin, and our data thus suggest that Hir proteins may be required for the function of metazoan centromeres. Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Author(s): Blackwell C, Martin KA, Greenall A, Pidoux A, Allshire RC, Whitehall SK
Publication type: Article
Publication status: Published
Journal: Molecular and Cellular Biology
ISSN (print): 0270-7306
ISSN (electronic): 1067-8824
Publisher: American Society for Microbiology
PubMed id: 15121850
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