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Lookup NU author(s): Tibor Nagy
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The integrative system of phage 16-3 of Rhizobium meliloti 41 was shown to function in several bacterial species belonging to the Rhizobium, Bradyrhizobium, Azorhizobium, and Agrobacterium genera. It might also function in many other bacterial species provided that both the target site (attB) and the required host factor(s) are present. Here we report on the construction of a new integrative vector that can be utilized in gene regulation studies. It provides an opportunity to create a single-copy set-up for characterizing DNA-protein interactions in vivo, in a wide range of bacteria. To demonstrate the usefulness of the vector, transcription repression by binding of the C repressor protein of phage 16-3 to wild type operators was studied. The assay system provided highly reproducible quantitative data on repression. © 2004 Elsevier Inc. All rights reserved.
Author(s): Ferenczi S, Ganyu A, Blaha B, Semsey S, Nagy T, Csiszovszki Z, Orosz L, Papp PP
Publication type: Article
Publication status: Published
ISSN (print): 0147-619X
ISSN (electronic): 1095-9890
Publisher: Academic Press
PubMed id: 15212892
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