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CDK1/cyclin B regulation during oocyte maturation in two closely related lugworm species, Arenicola marina and Arenicola defodiens

Lookup NU author(s): Dr Fabienne Chausson, Lesley Paterson, Dr Katherine Betteley, Lucie Hannah, Emeritus Professor Matt Bentley


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The molecular mechanisms underlying oocyte maturation in the annelid polychaetes Arenicola marina and Arenicola defodiens were investigated. In both species, a hitherto unidentified hormone triggers synchronous and rapid transition from prophase to metaphase, a maturation process which can be easily reproduced in vitro. Activation of a roscovitine- and olomoucine-sensitive M-phase-specific histone, H1 kinase, occurs during oocyte maturation. Using affinity chromatography on immobilized p9CKshs1, we purified CDK1 and cyclin B from oocyte extracts prepared from both phases and both species. In prophase, CDK1 is present both as an inactive, but Thr161-phosphorylated monomer, and as an inactive (Tyr15-phosphorylated) heterodimer with cyclin B. Prophase to metaphase transition is associated with complete tyrosine dephosphorylation of the cyclin B-associated CDK1, with phosphorylation of cyclin B, and with dramatic activation of the kinase activity of the CDK1/ cyclin B complex. We propose that Arenicola oocytes may provide an ideal model system to investigate the acquisition of the ability of oocytes to be fertilized that occurs as oocyte shift from prophase to metaphase, an important physiological event, probably regulated by active CDK1/cyclin B.

Publication metadata

Author(s): Chausson F, Paterson LA, Betteley KA, Hannah L, Meijer L, Bentley MG

Publication type: Article

Publication status: Published

Journal: Development Growth and Differentiation

Year: 2004

Volume: 46

Issue: 1

Pages: 71-82

ISSN (print): 0012-1592

Publisher: Wiley-Blackwell


DOI: 10.1111/j.1440-169X.2004.00723.x

PubMed id: 15008856


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