Toggle Main Menu Toggle Search

Open Access padlockePrints

MICA intron 1 sequences of conserved extended HLA haplotypes: Implications for sequencing-based typing

Lookup NU author(s): Dr Tevfik Dorak


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


The human major histocompatibility complex (MHC) class I chain-related gene A (MICA) has a high degree of genetic diversity. Several methods have been used in MICA typing. Recent studies reported different results for the same reference cell lines typed by different methods. By searching the GenBank, we found an indel polymorphism in MICA intron 1 corresponding to the area where one of the sequencing-based typing primers used by others is located. We investigated this polymorphism in 43 reference samples by primer cycle sequencing. This approach revealed three haplotype-specific patterns of polymorphisms in intron 1. This study provided evidence that one of the primers commonly used in MICA typing may fail to amplify both alleles in certain heterozygous combinations. Our data showed a correlation between the three patterns in MICA intron 1 and exon 5 short tandem repeat (STR) alleles. Being neutral ones, the intron 1 and STR polymorphisms appeared to mark the ancestral lineages better than the coding region polymorphisms. © 2004 Nature Publishing Group All rights reserved.

Publication metadata

Author(s): Shao W, Lobashevsky ES, Kaslow RA, Dorak MT

Publication type: Article

Publication status: Published

Journal: Genes and Immunity

Year: 2004

Volume: 5

Issue: 5

Pages: 371-374

ISSN (print): 1466-4879

ISSN (electronic): 1476-5470

Publisher: Nature Publishing Group


DOI: 10.1038/sj.gene.6364103


Altmetrics provided by Altmetric