Toggle Main Menu Toggle Search

Open Access padlockePrints

Simultaneous intra- and extracellular superoxide monitoring using an integrated optical and electrochemical sensor system

Lookup NU author(s): Dr Seung Chang, Dr James Henderson, Emeritus Professor Calum McNeilORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


A new integrated optical and electrochemical sensor system for simultaneous monitoring of intra- and extracellular superoxide (O2-) was developed using an array-based cell chip. For in vitro assays, A172 human glioblastoma cells were transferred into the cell chip and stimulated by phorbol 12-myristate 13-acetate (PMA). Intracellular O2- generation was detected via fluorescence image analysis with a dye probe, dihydrorhodamine 123 (DHR 123). Extracellular O2- was detected using an amperometric sensor constructed by immobilisation of cytochrome c using a binder, 3,3′-dithiobis(sulphosuccinimidylpropionate), to attach the redox protein onto the surface of electrodeposited Au electrodes incorporated into the optically transparent cell chip. The simultaneous intra- and extracellular production of O2- was successfully observed from PMA-stimulated A172 cells and inhibited by superoxide dismutase (SOD). The quantification of O2- concentration based on a mathematical model study and possible applications using the sensor system developed were discussed. The results confirm that there was no detectable interference or crosstalk between the optical and electrochemical assays. Feasibility of the integration of the two methods, optical and electrochemical, and the neutralisation of the intra- and extracellular O2- levels by SOD have been demonstrated. © 2004 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Chang S-C, Rodrigues NP, Zurgil N, Henderson JR, Bedioui F, McNeil CJ, Deutsch M

Publication type: Article

Publication status: Published

Journal: Biochemical and Biophysical Research Communications

Year: 2005

Volume: 327

Issue: 4

Pages: 979-984

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press


DOI: 10.1016/j.bbrc.2004.12.108

PubMed id: 15652491


Altmetrics provided by Altmetric