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DNA binding of a molecular-scale receptor in the presence of zinc(II) ions

Lookup NU author(s): Professor Andrew Benniston, Emeritus Professor Anthony Harriman, Donald Lawrie, Maryam Mehrabi

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Abstract

The properties of a tritopic artificial biological probe are described. This probe consists of a luminescent pyrene-thiophene unit connected by an ethynylene group to a 2,2′:6′,2″-terpyridine (terpy) cation binding site. The pyrene unit, as evidenced by fluorescence spectroscopy under illumination at 400 nm, is capable of intercalating into double-stranded calf-thymus DNA in H2O (buffered, pH = 7.0) at 25 °C. The binding constant K was calculated to be 6.0 × 105M-1. Titration of zinc(II) ions in an aqueous (pH = 7.0) solution containing the intercalated probe results in fluorescence quenching which again is a consequence of the zinc(II) ions binding to the terpy site. The DNA-bound probe has also been shown to undergo singlet energy transfer to intercalated ethidium bromide with a rate constant of 9.4 × 109 s-1. © Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005.


Publication metadata

Author(s): Benniston AC, Harriman A, Lawrie DJ, Mehrabi M

Publication type: Article

Publication status: Published

Journal: European Journal of Organic Chemistry

Year: 2005

Issue: 7

Pages: 1384-1391

Print publication date: 01/04/2005

ISSN (print): 1434-193X

ISSN (electronic): 1099-0690

Publisher: Wiley

URL: http://dx.doi.org/10.1002/ejoc.200400813

DOI: 10.1002/ejoc.200400813


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