Browse by author
Lookup NU author(s): Dr Jun-yong Huang
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Compromising the activity of the spindle checkpoint permits mitotic exit in the presence of unattached kinetochores and, consequently, greatly increases the rate of aneuploidy in the daughter cells [1-3]. The metazoan checkpoint mechanism is more complex than in yeast in that it requires additional proteins and activities besides the classical Mads and Bubs. Among these are Rod, Zw10, and Zwilch, components of a 700 Kdal complex (Rod/Zw10) [4-6] that is required for recruitment of dynein/dynactin to kinetochores [7, 8] but whose role in the checkpoint is poorly understood. The dynamics of Rod and Mad2, examined in different organisms, show intriguing similarities as well as apparent differences [7, 9]. Here we simultaneously follow GFP-Mad2 and RFP-Rod and find they are in fact closely associated throughout early mitosis. They accumulate simultaneously on kinetochores and are shed together along microtubule fibers after attachment. Their behavior and position within attached kinetochores is distinct from that of BubR1; Mad2 and Rod colocalize to the outermost kinetochore region (the corona), whereas BubR1 is slightly more interior. Moreover, Mad2, but not BubR1, Bub1, Bub3, or Mps1, requires Rod/Zw10 for its accumulation on unattached kinetochores. Rod/Zw10 thus contributes to checkpoint activation by promoting Mad2 recruitment and to checkpoint inactivation by recruiting dynein/dynactin that subsequently removes Mad2 from attached kinetochores. ©2005 Elsevier Ltd. All rights reserved.
Author(s): Buffin E, Lefebvre C, Huang J, Gagou ME, Karess RE
Publication type: Article
Publication status: Published
Journal: Current Biology
ISSN (print): 0960-9822
ISSN (electronic): 1879-0445
Publisher: Cell Press
PubMed id: 15886105
Altmetrics provided by Altmetric