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Study of apoptosis and heat shock protein (HSP) expression in hepatocytes following radiofrequency ablation (RFA)

Lookup NU author(s): Dr Claire Richardson, Emeritus Professor Paul FlecknellORCiD, Dr Helen Robertson, Professor Alastair BurtORCiD, Professor Derek Manas


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Background. Radiofrequency ablation (RFA) of the liver produces necrosis of the hepatocytes. Histological examination shows a sharp demarcation between ablated and normal liver tissue. This experiment was carried out to study the cellular injury produced by RFA in area surrounding the ablated tissue and effect of reperfusion on this zone. Material and Methods. Five pigs underwent RFA of liver parenchyma. Four pigs were sacrificed 30 min after RFA and one pig was sacrificed 5 days later. Ablated lesions including surrounding liver parenchyma was examined for apoptosis and HSP 70 expression. Results. There was a zone of transition surrounding the necrotic ablated area that showed apoptosis as well as increased HSP 70 expression. This was more prevalent in the pig that was sacrificed 5 days later. Conclusion. RFA produces sub lethal injury in the zone of transition causing apoptosis and increase in HSP 70 expression. Increased HSP expression enhances immunogenicity of these cells that can have therapeutic implications for the treatment of liver. © 2005 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Rai R, Richardson C, Flecknell P, Robertson H, Burt A, Manas DM

Publication type: Article

Publication status: Published

Journal: Journal of Surgical Research

Year: 2005

Volume: 129

Issue: 1

Pages: 147-151

Print publication date: 01/11/2005

ISSN (print): 0022-4804

ISSN (electronic): 1095-8673

Publisher: Academic Press


DOI: 10.1016/j.jss.2005.03.020

PubMed id: 15975593


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