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Mast cells and c-Kit expression in liver allograft rejection

Lookup NU author(s): Dr Ahmed Ahmed El-Refaie, Professor Alastair BurtORCiD


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Aims: The pathogenesis of rejection following liver transplantation is not fully understood. It has been postulated that mast cells may play a role in acute and chronic rejection of a number of other solid organ grafts. The aim of this study was to assess the possible role of mast cells and c-Kit+ cells in acute and chronic liver allograft rejection. Methods and results: Biopsy specimens from (i) 'time zero' grafts with a minimal degree of perfusion injury (controls), (ii) transplanted livers with different grades of acute rejection, and (iii) transplanted livers with end-stage chronic rejection, were stained immunohistochemically using monoclonal anti-mast cell tryptase and polyclonal anti-c-Kit antibodies. Tryptase- and c-Kit-positive cell densities were assessed by image analysis. Tryptase-postive mast cell densities (P < 0.001) were strongly correlated with acute liver allograft rejection grades and chronic liver allograft rejection. Furthermore, a similarly strong relationship was found between c-Kit+ cell densities and increasing rejection grade (P < 0.001). Conclusions: Tryptase- and c-Kit-positive mast cells form part of the inflammatory infiltrate in both acute and chronic liver allograft rejection, and may be important effector cells in these processes. © 2005 Blackwell Publishing Limited.

Publication metadata

Author(s): Ahmed El-Refaie AM, Burt AD

Publication type: Article

Publication status: Published

Journal: Histopathology

Year: 2005

Volume: 47

Issue: 4

Pages: 375-381

ISSN (print): 0309-0167

ISSN (electronic): 1365-2559

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1111/j.1365-2559.2005.02239.x

PubMed id: 16178892


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