Toggle Main Menu Toggle Search

Open Access padlockePrints

Treadmill exercise in claudicants on aspirin results in improved antioxidant status but only minimal platelet activation

Lookup NU author(s): Dr Jonathan Smout, Dr Brian Shenton, Professor Gerard Stansby

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The consequence of exercise on platelets remains controversial and adverse effects may result from repeated ischaemia reperfusion injury. We investigated platelet activation (platelet P-selectin (PS), and activated glycoprotein (Gp) IIb/IIIa), platelet-monocyte aggregates (PMA) and total plasma antioxidant status (TPAS) in claudicants after exercise. Twenty claudicants, taking 75 mg of aspirin daily, were subjected to repeated treadmill testing (3 km/h, 10% inclination). Blood was sampled before and after exercise. Activated GpIIb/IIIa, PS and PMA were quantified with flow cytometry. TPAS was quantified using a decolourisation assay. Percent positive cells for PS (pre-exercise 3.76% vs. 40 min post-exercise 4.10%; P<0.05) and platelet-monocyte aggregates (pre-exercise: 25.31% vs. 40min post-exercise 26.99%; P<0.05) were significantly higher after exercise. Relative median fluorescence (RMF) for activated GpIIb/IIIa was significantly higher 40min after exercise (pre-exercise: 3.04 vs. 40 min post-exercise: 4.01; P<0.05). TPAS was significantly higher post-exercise (pre-exercise: 1.31 mmol/1 vs. 1 min post-exercise: 1.40 mmol/l and 40 min post-exercise: 1.38 mmol/1; P< 0.01). Following moderate exercise, 'aspirin treated claudicants' show marginal platelet activation, PMA formation and a favourable improvement in antioxidant status. Further studies are required to assess the effect of additional antiplatelet agents and the significance of platelet-monocyte interactions. The possibility that aspirin contributes to the TPAS changes following exercise needs to be investigated. © 2005 Taylor & Francis.


Publication metadata

Author(s): Cleanthis M, Smout J, Bhattacharya V, Ashour H, Shenton B, Stansby G

Publication type: Article

Publication status: Published

Journal: Platelets

Year: 2005

Volume: 16

Issue: 8

Pages: 446-452

ISSN (print): 0953-7104

ISSN (electronic): 1369-1635

Publisher: Informa Healthcare

URL: http://dx.doi.org/10.1080/09537100500128740

DOI: 10.1080/09537100500128740

PubMed id: 16287611


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share