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Treadmill exercise in claudicants on aspirin results in improved antioxidant status but only minimal platelet activation

Lookup NU author(s): Dr Jonathan Smout, Dr Brian Shenton, Professor Gerard Stansby


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The consequence of exercise on platelets remains controversial and adverse effects may result from repeated ischaemia reperfusion injury. We investigated platelet activation (platelet P-selectin (PS), and activated glycoprotein (Gp) IIb/IIIa), platelet-monocyte aggregates (PMA) and total plasma antioxidant status (TPAS) in claudicants after exercise. Twenty claudicants, taking 75 mg of aspirin daily, were subjected to repeated treadmill testing (3 km/h, 10% inclination). Blood was sampled before and after exercise. Activated GpIIb/IIIa, PS and PMA were quantified with flow cytometry. TPAS was quantified using a decolourisation assay. Percent positive cells for PS (pre-exercise 3.76% vs. 40 min post-exercise 4.10%; P<0.05) and platelet-monocyte aggregates (pre-exercise: 25.31% vs. 40min post-exercise 26.99%; P<0.05) were significantly higher after exercise. Relative median fluorescence (RMF) for activated GpIIb/IIIa was significantly higher 40min after exercise (pre-exercise: 3.04 vs. 40 min post-exercise: 4.01; P<0.05). TPAS was significantly higher post-exercise (pre-exercise: 1.31 mmol/1 vs. 1 min post-exercise: 1.40 mmol/l and 40 min post-exercise: 1.38 mmol/1; P< 0.01). Following moderate exercise, 'aspirin treated claudicants' show marginal platelet activation, PMA formation and a favourable improvement in antioxidant status. Further studies are required to assess the effect of additional antiplatelet agents and the significance of platelet-monocyte interactions. The possibility that aspirin contributes to the TPAS changes following exercise needs to be investigated. © 2005 Taylor & Francis.

Publication metadata

Author(s): Cleanthis M, Smout J, Bhattacharya V, Ashour H, Shenton B, Stansby G

Publication type: Article

Publication status: Published

Journal: Platelets

Year: 2005

Volume: 16

Issue: 8

Pages: 446-452

ISSN (print): 0953-7104

ISSN (electronic): 1369-1635

Publisher: Informa Healthcare


DOI: 10.1080/09537100500128740

PubMed id: 16287611


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