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The in vitro effects of triiodothyronine on epidermal growth factor-induced trophoblast function

Lookup NU author(s): Dr Judith Bulmer


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The development of the human placenta involves a complex process of tightly regulated proliferation and invasion by extravillous trophoblast into the uterine decidua. Inadequate placentation is a feature of intrauterine growth restriction and other gestational pathology. There is some evidence that T 3 plays a role in the regulation of these processes and that T 3 may act synergistically with epidermal growth factor (EGF). The aim of this study was to define the expression of thyroid hormone receptors in extravillous trophoblast, elucidate the effects of T3 on both proliferation and differentiation of human trophoblast cells of varying origins, and define the potential interaction between EGF and T3 on these processes. Using immunohistochemistry, specific thyroid hormone receptor isoforms were localized in extravillous trophoblast in first- and second-trimester placental bed biopsies, indicating potential sensitivity to T3. In studies of human trophoblast-derived cell lines and primary cultures of cytotrophoblast cells in vitro, T3 and EGF exerted an antiproliferative effect on an extravillous-like cell line (SGHPL-4) but stimulated proliferation in JEG-3 choriocarcinoma cells. EGF enhanced survival of nonproliferative term primary cytotrophoblast cells and significantly enhanced invasion of fibrin gels by SGHPL-4 cells, an effect attenuated by T3. Both T3 and EGF also significantly enhanced SGHPL-4 motility. These results suggest that EGF and T3 may act synergistically to regulate both proliferation and differentiated function of human trophoblast. Copyright © 2005 by The Endocrine Society.

Publication metadata

Author(s): Barber KJ, Franklyn JA, McCabe CJ, Khanim FL, Bulmer JN, Whitley GSJ, Kilby MD

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Endocrinology and Metabolism

Year: 2005

Volume: 90

Issue: 3

Pages: 1655-1661

Print publication date: 01/03/2005

ISSN (print): 0021-972X

ISSN (electronic): 1945-7197

Publisher: The Endocrine Society


DOI: 10.1210/jc.2004-0785

PubMed id: 15598697


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