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Lookup NU author(s): Dr Radka Philipova, Dr Jolanta Kisielewska, Dr Pin Lu, Mark Larman, Dr Jun-yong Huang, Emeritus Professor Michael Whitaker
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Fertilization of sea urchin eggs results in a large, transient increase in intracellular free Ca2+ concentration that is responsible for re-initiation of the cell division cycle. We show that activation of ERK1, a Ca2+-dependent MAP kinase response, is required for both DNA synthesis and cell cycle progression after fertilization. We combine experiments on populations of cells with analysis at the single cell level, and develop a proxy assay for DNA synthesis in single embryos, using GFP-PCNA. We compare the effects of low molecular weight inhibitors with a recombinant approach targeting the same signalling pathway. We find that inhibition of the ERK pathway at fertilization using either recombinant ERK phosphatase or U0126, a MEK inhibitor, prevents accumulation of GFP-PCNA in the zygote nucleus and that U0126 prevents incorporation of [3H]-thymidine into DNA. Abrogation of the ERK1 signalling pathway also prevents chromatin decondensation of the sperm chromatin after pronuclear fusion, nuclear envelope breakdown and formation of a bipolar spindle.
Author(s): Philipova R, Kisielewska J, Lu P, Larman M, Huang J-Y, Whitaker M
Publication type: Article
Publication status: Published
Journal: Development
Year: 2005
Volume: 132
Issue: 3
Pages: 579-589
ISSN (print): 0950-1991
ISSN (electronic): 1477-9129
Publisher: The Company of Biologists Ltd.
URL: http://dx.doi.org/10.1242/dev.01607
DOI: 10.1242/dev.01607
PubMed id: 15634691
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