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Expression and interaction of the transcriptional coregulators, CBP/p300, in the human myometrium during pregnancy and labor

Lookup NU author(s): Dr Neil Chapman, Barbara Innes, Professor Nick Europe-Finner, Professor Steve Robson

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Abstract

OBJECTIVE: In humans, the factors that govern the switch from myometrial quiescence to coordinated contractions at the initiation of labor are not well defined. Recent studies have highlighted a role for the coactivator, CREB binding protein (CBP), in the human myometrium during pregnancy and labor through its ability to acetylate histones. In the present study, the expression of CBP and its related coactivator, p300, were examined. METHODS: Levels and interactions of CBP and its paralogue p300 were determined by Western blotting, immunohistochemistry, and coimmunoprecipitation experiments using myometrial biopsy samples from nonpregnant (NP), pregnant nonlaboring (P), and spontaneously laboring (SL) women. RESULTS: Levels of CBP were seen to increase in term P myometrial samples but were then greatly reduced in SL myometrium. In contrast, levels of p300 remained uniform between NP, P, and SL tissues. These observations were confirmed by immunhistochemical analyses. Immunoprecipitation experiments highlighted that CBP was able to interact with CREB, CREM, ATF-2, and p300 in P lower segment myometrium. CONCLUSION: Recent evidence suggests that competition for CBP plays an important role in regulating gene expression during cell growth. Consequently our data suggest that the increase in myometrial CBP levels during pregnancy may occur to meet this increase in CBP demand. Moreover, from coimmunoprecipitation experiments, this increase in CBP expression would be expected to facilitate the transactivation potential of the cyclic adenosine monophosphate (cAMP)-dependent transcription factors CREB, CREM, and ATF-2. Copyright © 2005 by the Society for Gynecologic Investigation.


Publication metadata

Author(s): Long AA, Chapman NR, Innes B, Europe-Finner GN, Robson SC

Publication type: Article

Publication status: Published

Journal: Journal of the Society for Gynecologic Investigation

Year: 2005

Volume: 12

Issue: 2

Pages: 92-97

Print publication date: 01/02/2005

ISSN (print): 1933-7191

ISSN (electronic): 1933-7205

Publisher: Sage Publications

URL: http://dx.doi.org/10.1016/j.jsgi.2004.10.012

DOI: 10.1016/j.jsgi.2004.10.012

PubMed id: 15695103


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