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Accumulation and distribution of neutral lipid droplets is non-uniform in ovine blastocysts produced in vitro in either the presence or absence of serum

Lookup NU author(s): Dr Alexandra Reis


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Sheep zygotes were cultured in serum-free or serum-supplemented media to determine effects on blastocyst yields and within-blastocyst abundance and distribution of neutral lipid droplets. Embryos cultured in synthetic oviduct fluid supplemented with bovine serum albumin (0.4% w/v) (SBSA) generated similar blastocyst yields (mean ± s.e.m. = 20% ± 5) to those in synthetic oviduct fluid supplemented with serum (10% v/v) from ewes fed a diet containing 0% (SZFO; 26% ± 2) or 3% fish oil (S3FO; 23% ± 3). SBSA zygotes generated more good-quality blastocysts than their SZFO or S3FO counterparts (P < 0.05). Within-blastocyst abundance of neutral lipid droplets was non-uniform; data were collected from discrete embryo sectors (each = 2700 μm2) representing highest (H), intermediate (I) and lowest (L) densities of accumulation. For all sectors, area (μm2) occupied by lipid droplets in SBSA blastocysts (mean H = 470; I = 370; L = 245) was smaller (P < 0.01) than occupied in others (SBSA:SZFO=1:1.41, 1:1.48 and 1:1.42; SBSA:S3FO=1:1.36, 1:1.30 and 1:1.31; data for H, I and L, respectively). Among S3FO blastocysts only, inferior quality was associated with greater lipid abundance. Overall, embryo culture in the presence of serum increased neutral lipid droplet abundance but accumulation was non-uniform. © CSIRO 2005.

Publication metadata

Author(s): Reis A, McCallum GJ, McEvoy TG

Publication type: Article

Publication status: Published

Journal: Reproduction, Fertility and Development

Year: 2005

Volume: 17

Issue: 8

Pages: 815-823

Print publication date: 01/01/2005

ISSN (print): 1031-3613

ISSN (electronic): 1448-5990

Publisher: C S I R O Publishing


DOI: 10.1071/RD05017

PubMed id: 16476209


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