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Lookup NU author(s): Dr Alexandra Reis
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Sheep zygotes were cultured in serum-free or serum-supplemented media to determine effects on blastocyst yields and within-blastocyst abundance and distribution of neutral lipid droplets. Embryos cultured in synthetic oviduct fluid supplemented with bovine serum albumin (0.4% w/v) (SBSA) generated similar blastocyst yields (mean ± s.e.m. = 20% ± 5) to those in synthetic oviduct fluid supplemented with serum (10% v/v) from ewes fed a diet containing 0% (SZFO; 26% ± 2) or 3% fish oil (S3FO; 23% ± 3). SBSA zygotes generated more good-quality blastocysts than their SZFO or S3FO counterparts (P < 0.05). Within-blastocyst abundance of neutral lipid droplets was non-uniform; data were collected from discrete embryo sectors (each = 2700 μm2) representing highest (H), intermediate (I) and lowest (L) densities of accumulation. For all sectors, area (μm2) occupied by lipid droplets in SBSA blastocysts (mean H = 470; I = 370; L = 245) was smaller (P < 0.01) than occupied in others (SBSA:SZFO=1:1.41, 1:1.48 and 1:1.42; SBSA:S3FO=1:1.36, 1:1.30 and 1:1.31; data for H, I and L, respectively). Among S3FO blastocysts only, inferior quality was associated with greater lipid abundance. Overall, embryo culture in the presence of serum increased neutral lipid droplet abundance but accumulation was non-uniform. © CSIRO 2005.
Author(s): Reis A, McCallum GJ, McEvoy TG
Publication type: Article
Publication status: Published
Journal: Reproduction, Fertility and Development
Year: 2005
Volume: 17
Issue: 8
Pages: 815-823
Print publication date: 01/01/2005
ISSN (print): 1031-3613
ISSN (electronic): 1448-5990
Publisher: C S I R O Publishing
URL: http://dx.doi.org/10.1071/RD05017
DOI: 10.1071/RD05017
PubMed id: 16476209
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