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Flash pyrolysis - A rapid method for screening bacterial species for the presence of bacteriohopanepolyols

Lookup NU author(s): Mark Sugden, Dr Helen Talbot, Dr Paul Farrimond

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Abstract

Flash pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) was applied to freeze dried cells of eleven bacterial species. Six species known to synthesise bacteriohopanepolyols (BHPs) were found to produce easily identifiable C27-C35 hopenes and hopanes upon flash pyrolysis. These compounds could not be observed in the pyrolysis products of five species, two of which are known not to produce BHPs. In the search for BHP-containing bacteria this rapid screening approach prevents unnecessary time-consuming laboratory work to characterise species that would produce negative results. © 2005 Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Sugden MA, Talbot HM, Farrimond P

Publication type: Article

Publication status: Published

Journal: Organic Geochemistry

Year: 2005

Volume: 36

Issue: 6

Pages: 975-979

Print publication date: 01/06/2005

ISSN (print): 0146-6380

ISSN (electronic): 1873-5290

Publisher: Pergamon

URL: http://dx.doi.org/10.1016/j.orggeochem.2004.12.003

DOI: 10.1016/j.orggeochem.2004.12.003

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