ePrints

Functional analysis of 11 putative essential genes in Bacillus subtilis

Lookup NU author(s): Professor Jeff ErringtonORCiD

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Systematic inactivation of Bacillus subtilis genes has previously revealed that 271 are indispensable for growth. In the present study, 11 of these (YacA, ydiB, ydiC, ykqC, ylaN, yloQ, ymdA, yneS, yqel, yqjK and ywlC) were identified as genes encoding proteins of unknown function. By analysing the effects of protein depletion, and examining the subcellular localization of these proteins, a start has been made in elucidating their functions. It was found that four of these genes (ydiB, yloQ, yqel and ywlC) were not required for B. subtilis viability. Analysis of the localization of YkqC suggests that it co-localizes with ribosomes, and it is proposed that it is involved in processing either rRNA or specific mRNAs when they are associated with the ribosome. The results suggest that other novel essential proteins may be involved in lipid synthesis and control of cell wall synthesis. © 2006 SGM.

Publication metadata

Author(s): Hunt A, Rawlins JP, Thomaides HB, Errington J

Publication type: Article

Publication status: Published

Journal: Microbiology

Year: 2006

Volume: 152

Issue: 10

Pages: 2895-2907

Print publication date: 01/10/2006

ISSN (print): 1350-0872

ISSN (electronic): 1465-2080

Publisher: Society for General Microbiology

URL: http://dx.doi.org/10.1099/mic.0.29152-0

DOI: 10.1099/mic.0.29152-0

Altmetrics

Share