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The flagellar-specific transcription factor, σ28, is the Type III secretion chaperone for the flagellar-specific anti- σ28 factor FlgM

Lookup NU author(s): Dr Phillip AldridgeORCiD, Christine Aldridge, Christopher Birchall

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Abstract

The σ28 protein is a member of the bacterial σ70-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The σ28 protein is regulated in response to flagellar assembly by the anti-σ28 factor FlgM. FlgM inhibits σ28-dependent transcription of genes whose products are needed late in assembly until the flagellar basal motor structure, the hook-basal body (HBB), is constructed. A second function for the σ28 transcription factor has been discovered: σ28 facilitates the secretion of FlgM through the HBB, acting as the FlgM Type III secretion chaperone. Transcription-specific mutants in σ28 were isolated that remained competent for FlgM-facilitated secretion separating the transcription and secretion-facilitation activities of σ28. Conversely, we also describe the isolation of mutants in σ28 that are specific for FlgM-facilitated secretion. The data demonstrate that σ28 is the Type III secretion chaperone for its own anti-sigma factor FlgM. Thus, a novel role for a σ70- family transcription factor is described. © 2006 by Cold Spring Harbor Laboratory Press.

Publication metadata

Author(s): Aldridge PD, Karlinsey JE, Aldridge C, Birchall C, Thompson D, Yagasaki J, Hughes KT

Publication type: Article

Publication status: Published

Journal: Genes & Development

Year: 2006

Volume: 20

Issue: 16

Pages: 2315-2326

Print publication date: 15/08/2006

ISSN (print): 0890-9369

ISSN (electronic): 1549-5477

Publisher: Cold Spring Harbor Laboratory Press

URL: http://dx.doi.org/10.1101/gad.380406

DOI: 10.1101/gad.380406

PubMed id: 16912280

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