Browse by author
Lookup NU author(s): Frances Davison, Dr Jon Aust, Dr Jane Falconer, Professor Colin Brooks
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Using a novel mAb specific for mouse Ly49B, we report here that Ly49B, the last remaining member of the C57 Ly49 family to be characterized, is expressed at low levels on ∼1.5% of spleen cells, none which are NK cells or T cells but which instead belong to several distinct subpopulations of myeloid cells defined by expression of CD11b and different levels of Gr1. Much larger proportions of bone marrow and peritoneal cells expressed Ly49B, all being CD11b+ and comprising multiple subpopulations defined by light scatter, F4/80, and Gr1 expression. Costaining for Ly49Q, also expressed on myeloid cells, revealed that Ly49B and Ly49Q were most strongly expressed on nonoverlapping subpopulations, Ly49Qhigh cells being mostly B220 +CD4+ and/or CD8+, Ly49B+ cells lacking these markers. Myeloid populations that developed from bone marrow progenitors in vitro frequently coexpressed both Ly49B and Ly49Q, and Ly49B expression could be up-regulated by LPS, α-IFN, and γ-IFN, often independently of Ly49Q. PCR analysis revealed that cultured NK cells and T cells contained Ly49B transcripts, and Ly49B expression could be detected on NK cells cultured in IL-12 plus IL-18, and on an immature NK cell line. Immunohistochemical studies showed that Ly49B expression in tissues overlapped with but was distinct from that of all other myeloid molecules examined, being particularly prominent in the lamina propria and dome of Peyer's patches, implicating an important role of Ly49B in gut immunobiology. In transfected cells, Ly49B was found to associate with SHP-1, SHP-2, and SHIP in a manner strongly regulated by intracellular phosphorylation events. Copyright © 2006 by The American Association of Immunologists, Inc.
Author(s): Gays F, Aust JG, Reid DM, Falconer J, Toyama-Sorimachi N, Taylor PR, Brooks CG
Publication type: Article
Publication status: Published
Journal: Journal of Immunology
Year: 2006
Volume: 177
Issue: 9
Pages: 5840-5851
Print publication date: 01/11/2006
Acceptance date: 25/07/2006
ISSN (print): 0022-1767
ISSN (electronic): 1550-6606
Publisher: American Association of Immunologists
URL: http://dx.doi.org/10.4049/jimmunol.177.9.5840
DOI: 10.4049/jimmunol.177.9.5840
PubMed id: 17056508
Altmetrics provided by Altmetric
