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Lookup NU author(s): Dr Alexandra Reis,
Dr Suzanne Madgwick,
Professor Keith Jones
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Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.
Author(s): Gorr IH, Reis A, Boos D, Wuhr M, Madgwick S, Jones KT, Stemmann O
Publication type: Article
Publication status: Published
Journal: Nature Cell Biology
ISSN (print): 1465-7392
ISSN (electronic): 1476-4679
Publisher: Nature Publishing Group
PubMed id: 16906143
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