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Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media

Lookup NU author(s): Geok Yuan Annie Tan, Emeritus Professor Alan Ward, Professor Michael Goodfellow

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Abstract

Large numbers of actinomycetes provisionally assigned to the genus Amycolatopsis were isolated from soil samples using a dilution plate procedure and three novel media designed to be selective for members of this genus. A set of genus-specific oligonucleotide primers developed for the rapid identification of unknown Amycolatopsis strains was used to determine whether representative strains taken from the selective isolation plates produced the diagnostic amplification product. The 175 isolates which tested positive were assigned to major, minor and single-membered colour-groups. Representatives of the major colour-groups had a profile of chemical and morphological properties consistent with their assignment to the genus Amycolatopsis; strains taken to represent most of these taxa formed distinct phyletic lines in the Amycolatopsis 16S rRNA gene tree. Many of the representative isolates were closely related to either Amycolatopsis mediterranei or to Amycolatopsis orientalis or formed new evolutionary lines in either the Amycolatopsis orientalis or Amycolatopsis methanolica 16S rRNA subclades. It is evident that the genus Amycolatopsis is grossly underspeciated. © 2006 Elsevier GmbH. All rights reserved.

Publication metadata

Author(s): Tan GYA, Ward AC, Goodfellow M

Publication type: Article

Publication status: Published

Journal: Systematic and Applied Microbiology

Year: 2006

Volume: 29

Issue: 7

Pages: 557-569

ISSN (print): 0723-2020

ISSN (electronic): 1618-0984

Publisher: Urban und Fischer

URL: http://dx.doi.org/10.1016/j.syapm.2006.01.007

DOI: 10.1016/j.syapm.2006.01.007

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