Browse by author
Lookup NU author(s): Professor Janet Quinn
The resistance of Candida albicans to many stresses is dependent on the stress-activated protein kinase (SAPK) Hog1. Hence we have explored the role of Hog1 in the regulation of transcriptional responses to stress. DNA microarrays were used to characterize the global transcriptional responses of HOG1 and hog1 cells to three stress conditions that activate the Hog1 SAPK: osmotic stress, oxidative stress, and heavy metal stress. This revealed both stress-specific transcriptional responses and a core transcriptional response to stress in C. albicans. The core transcriptional response was characterized by a subset of genes that responded in a stereotypical manner to all of the stresses analyzed. Inactivation of HOG1 significantly attenuated transcriptional responses to osmotic and heavy metal stresses, but not to oxidative stress, and this was reflected in the role of Hog1 in the regulation of C. albicans core stress genes. Instead, the Cap1 transcription factor plays a key role in the oxidative stress regulation of C. albicans core stress genes. Our data show that the SAPK network in C. albicans has diverged from corresponding networks in model yeasts and that the C. albicans SAPK pathway functions in parallel with other pathways to regulate the core transcriptional response to stress. © 2006 by The American Society for Cell Biology.
Author(s): Enjalbert B, Smith DA, Cornell MJ, Alam I, Nicholls S, Brown AJP, Quinn J
Publication type: Article
Publication status: Published
Journal: Molecular Biology of the Cell
Year: 2006
Volume: 17
Issue: 2
Pages: 1018-1032
Date deposited: 20/09/2010
ISSN (print): 1059-1524
ISSN (electronic): 1939-4586
Publisher: American Society for Cell Biology
URL: http://dx.doi.org/10.1091/mbc.E05-06-0501
DOI: 10.1091/mbc.E05-06-0501
PubMed id: 16339080
Altmetrics provided by Altmetric
