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An approach to prepare membrane proteins for single-molecule imaging

Lookup NU author(s): Sofian Daud, Professor Jeremy LakeyORCiD


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(Figure Presented) Observed from above: A cysteine mutation in a membrane protein enables it to be fixed in a defined orientation on flat gold surfaces (see picture, left). Subsequent coassembly with thiolipids produces clearly defined, immobilized proteins. Analysis by atomic force microscopy provides topographs of similar quality to those revealed from 2D crystals. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

Publication metadata

Author(s): Cisneros DA, Muller DJ, Daud SM, Lakey JH

Publication type: Article

Publication status: Published

Journal: Angewandte Chemie - International Edition

Year: 2006

Volume: 45

Issue: 20

Pages: 3252-3256

ISSN (print): 1433-7851

ISSN (electronic): 1521-3773

Publisher: Wiley - VCH Verlag GmbH & Co. KGaA


DOI: 10.1002/anie.200504506


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