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Characterization of lactic acid bacteria and other gut bacteria in pigs by a macroarraying method

Lookup NU author(s): Narut Thanantong, Emerita Professor Sandra Edwards, Dr Olivier Sparagano


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Lactic acid bacteria (LAB) consist of many genera, Gram-positive, and nonspore-forming micro-organisms; some members being used as probiotics while some others have negative effects on pig health. Bacterial species in the gastrointestinal tract can produce antibacterial substances, reduce serum cholesterol in their host, or can be responsible for growth reduction, diarrhea, and intestinal epithelial damage. It is therefore important for the pig industry to evaluate the impact of food and farm management on the presence of "good" or "bad" bacteria and the risk for consumers. This articles focuses on the molecular identification of gut microflora species following different diets given to pigs in UK and correlating the data on growth, health, and welfare. First of all, pig feces were individually collected from sows before and after farrowing and also from piglets before and after weaning over several months. Bacteria colonies were grown on MRS agar plates from feces and DNA was extracted (QIAamp DNA stool kit) and amplified using 16S rDNA (27f and 519r) primers. DNA sequencing and sequence alignment allowed us to identify species-specific zones, which were used as probes in a macroarray system also known as reverse line blot hybridization. Some probes were found to be species specific for the following species: Lactobacillus acidophilus, L. animalis, L. gallinarum, L. kitasanotis, L salivarius, Streptococcus alactolyticus, S. hyointestinalis, and Sarcina ventriculi. Actual studies are now focusing on the impact of diets of the microflora in different gut parts and at different stages of the animal's life. © 2006 New York Academy of Sciences.

Publication metadata

Author(s): Thanantong N, Edwards S, Sparagano OAE

Editor(s): Blouin, EF; Maillard, JC

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine

Year of Conference: 2006

Pages: 276-279

ISSN: 0077-8923

Publisher: Wiley-Blackwell Publishing Inc.


DOI: 10.1196/annals.1373.037

PubMed id: 17135526

Library holdings: Search Newcastle University Library for this item

: 1st

Series Title: Annals of the New York Academy of Sciences

ISBN: 9781573316378