Toggle Main Menu Toggle Search

Open Access padlockePrints

Testing the aminostratigraphy of fluvial archives: the evidence from intra-crystalline proteins within freshwater shells

Lookup NU author(s): Kirsty Penkman, Professor Darrel Maddy, Dr Matthew Collins


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Until recently few studies of amino acid racemization of fossil bivalves and gastropods collected from river terrace deposits in Europe were based on the analysis of the intra-crystalline fraction. Instead they were based on the epimerization (racemization) of a single amino acid, isoleucine, and its inter-conversion to alloisoleucine. This paper presents data from the analysis of the intra-crystalline fraction of the shells, using a preparation technique of sample bleaching to remove the leachable matrix, thus leaving a component that exhibits closed-system behaviour. Reverse-phase HPLC separation with fluorescence detection allows the interpretation of four amino acids in detail: aspartic acid, glutamic acid, alanine and valine. The intra-crystalline fraction offers greater potential for improved resolution, especially when combined with the analysis of multiple amino acid d/l values, which racemize at different rates. This is explored using three species of freshwater gastropods (Bithynia tentaculata and troschelii, Valvata piscinalis) and the bivalve Corbicula. Sites of different ages within the Lower Thames river terrace sequence are used as a stratigraphical framework, with samples from other southern UK sites providing supplementary evidence. The results indicate better resolution using the intra-crystalline fraction over that obtained using unbleached shells, with differentiation possible at sites of up to MIS 7 age. However, for older sites, although values are always higher, the separation is less successful. A species effect has been identified between the gastropod shells. Despite the analysis of intra-crystalline protein, amino acid data from Corbicula remain problematical. Preliminary data on the opercula from Bithynia indicate that better resolution is possible, particularly at older sites. © 2007 Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Penkman K, Preece R, Keen D, Maddy D, Schreve D, Collins M

Publication type: Article

Publication status: Published

Journal: Quaternary Science Reviews

Year: 2007

Volume: 26

Issue: 22-24

Pages: 2958-2969

Print publication date: 01/11/2007

ISSN (print): 0277-3791

ISSN (electronic): 1873-457X


DOI: 10.1016/j.quascirev.2007.06.034


Altmetrics provided by Altmetric