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Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D- Ala interaction

Lookup NU author(s): Professor Jeff Errington FRSORCiD


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The clinically important vancomycin antibiotic inhibits the growth of pathogens such as Staphylococcus aureus by blocking cell wall synthesis through specific recognition of nascent peptidoglycan terminating in D-Ala-D-Ala. Here, we demonstrate the ability of single-molecule atomic force microscopy with antibiotic-modified tips to measure the specific binding forces of vancomycin and to map individual ligands on living bacteria. The single-molecule approach presented here provides new opportunities for understanding the binding mechanisms of antibiotics and for exploring the architecture of bacterial cell walls. © 2007 American Chemical Society.

Publication metadata

Author(s): Gilbert Y, Deghorain M, Wang L, Xu B, Pollheimer PD, Gruber HJ, Errington J, Hallet B, Haulot X, Verbelen C, Hols P, Dufrene YF

Publication type: Article

Publication status: Published

Journal: Nano Letters

Year: 2007

Volume: 7

Issue: 3

Pages: 796-801

Print publication date: 01/03/2007

ISSN (print): 1530-6984

ISSN (electronic): 1530-6992

Publisher: American Chemical Society


DOI: 10.1021/nl0700853

PubMed id: 17316058


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