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Selectivity for D-lactate incorporation into the peptidoglycan precursors of Lactobacillus plantarum: Role of Aad, a VanX-like D-alanyl-D-alanine dipeptidase

Lookup NU author(s): Dr Richard DanielORCiD, Professor Jeff ErringtonORCiD



Lactobacillus plantarum produces peptidoglycan precursors ending in D-lactate instead of D-alanine, making the bacterium intrinsically resistant to vancomycin. The ligase Ddl of L. plantarum plays a central role in this specificity by synthesizing D-alanyl-D-lactate depsipeptides that are added to the precursor peptide chain by the enzyme MurF. Here we show that L. plantarum also encodes a D-Ala-D-Ala dipeptidase, Aad, which eliminates D-alanyl-D-alanine dipeptides that are produced by the Ddl ligase, thereby preventing their incorporation into the precursors. Although D-alanine-ended precursors can be incorporated into the cell wall, inactivation of Aad failed to suppress growth defects of L. plantarum mutants deficient in D-lactate-ended precursor synthesis. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Publication metadata

Author(s): Deghorain M, Goffin P, Fontaine L, Mainardi J-L, Daniel R, Errington J, Hallet B, Hols P

Publication type: Article

Publication status: Published

Journal: Journal of Bacteriology

Year: 2007

Volume: 189

Issue: 11

Pages: 4332-4337

Print publication date: 01/06/2007

ISSN (print): 0021-9193

ISSN (electronic): 1098-5530

Publisher: American Society for Microbiology


DOI: 10.1128/JB.01829-06

PubMed id: 17400741


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