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A comparative proteomic analysis of human and rat embryonic cerebrospinal fluid

Lookup NU author(s): Dr Steven LisgoORCiD, Emerita Professor Susan Lindsay

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Abstract

During vertebrate central nervous system development, the apical neuroepithelium is bathed with embryonic Cerebrospinal Fluid (e-CSF) which plays regulatory roles in cortical cell proliferation and maintenance. Here, we report the first proteomic analysis of human e-CSF and compare it to an extensive proteomic analysis of rat e-CSF. As expected, we identified a large collection of protease inhibitors, extracellular matrix proteins, and transport proteins in CSF. However, we also found a surprising suite of signaling and intracellular proteins not predicted by previous proteomic analysis. Some of the intracellular proteins are likely to represent the contents of microvesicles recently described within the CSF (Marzesco, A. M., et al. J. Cell Sci. 2005, 118 (Pt. 13), 2849-2858). Defining the rich composition of e-CSF will enable a greater understanding of its concerted actions during critical stages of brain development. © 2007 American Chemical Society.

Publication metadata

Author(s): Zappaterra MD, Lisgo SN, Lindsay S, Gygi SP, Walsh CA, Ballif BA

Publication type: Article

Publication status: Published

Journal: Journal of Proteome Research

Year: 2007

Volume: 6

Issue: 9

Pages: 3537-3548

ISSN (print): 1535-3893

ISSN (electronic): 1535-3907

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/pr070247w

DOI: 10.1021/pr070247w

PubMed id: 17696520

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