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Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks

Lookup NU author(s): Professor Jeff Errington FRSORCiD


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In bacteria, several salvage responses to DNA replication arrest culminate in reassembly of the replisome on inactivated forks to resume replication. The PriA DNA helicase is a prominent trigger of this replication restart process, preceded in many cases by a repair and/or remodeling of the arrested fork, which can be performed by many specific proteins. The mechanisms that target these rescue effectors to damaged forks in the cell are unknown. We report that the single-stranded DNA binding (SSB) protein is the key factor that links PriA to active chromosomal replication forks in vivo. This targeting mechanism determines the efficiency by which PriA reaches its specific DNA-binding site in vitro and directs replication restart in vivo. The RecG and RecQ DNA helicases, which are involved in intricate replication reactivation pathways, also associate with the chromosomal replication forks by similarly interacting with SSB. These results identify SSB as a platform for linking a 'repair toolbox' with active replication forks, providing a first line of rescue responses to accidental arrest. © 2007 European Molecular Biology Organization | All Rights Reserved.

Publication metadata

Author(s): Lecointe F, Serena C, Velten M, Costes A, McGovern S, Meile J-C, Errington J, Ehrlich SD, Noirot P, Polard P

Publication type: Article

Publication status: Published

Journal: EMBO Journal

Year: 2007

Volume: 26

Issue: 19

Pages: 4239-4251

ISSN (print): 0261-4189

ISSN (electronic): 1460-2075

Publisher: Nature Publishing Group


DOI: 10.1038/sj.emboj.7601848

PubMed id: 17853894


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