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Lookup NU author(s): Professor Jeff ErringtonORCiD
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In bacteria, several salvage responses to DNA replication arrest culminate in reassembly of the replisome on inactivated forks to resume replication. The PriA DNA helicase is a prominent trigger of this replication restart process, preceded in many cases by a repair and/or remodeling of the arrested fork, which can be performed by many specific proteins. The mechanisms that target these rescue effectors to damaged forks in the cell are unknown. We report that the single-stranded DNA binding (SSB) protein is the key factor that links PriA to active chromosomal replication forks in vivo. This targeting mechanism determines the efficiency by which PriA reaches its specific DNA-binding site in vitro and directs replication restart in vivo. The RecG and RecQ DNA helicases, which are involved in intricate replication reactivation pathways, also associate with the chromosomal replication forks by similarly interacting with SSB. These results identify SSB as a platform for linking a 'repair toolbox' with active replication forks, providing a first line of rescue responses to accidental arrest. © 2007 European Molecular Biology Organization | All Rights Reserved.
Author(s): Lecointe F, Serena C, Velten M, Costes A, McGovern S, Meile J-C, Errington J, Ehrlich SD, Noirot P, Polard P
Publication type: Article
Publication status: Published
Journal: EMBO Journal
Year: 2007
Volume: 26
Issue: 19
Pages: 4239-4251
ISSN (print): 0261-4189
ISSN (electronic): 1460-2075
Publisher: Nature Publishing Group
URL: http://dx.doi.org/10.1038/sj.emboj.7601848
DOI: 10.1038/sj.emboj.7601848
PubMed id: 17853894
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