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Lookup NU author(s): Dr Sushma Grellscheid,
Professor David Elliott
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The RBMY (RNA-binding motif gene on Y chromosome) protein encoded by the human Y chromosome is important for normal sperm development. Although its precise molecular RNA targets are unknown at present, it is suggested that human RBMY (hRBMY) participates in splicing in the testis. Using systematic evolution of ligands by exponential enrichment, we found that RNA stem-loops capped by a CA/UCAA pentaloop are high-affinity binding targets for hRBMY. Subsequent nuclear magnetic resonance structural determination of the hRBMY RNA recognition motif (RRM) in complex with a high-affinity target showed two distinct modes of RNA recognition. First, the RRM β-sheet surface binds to the RNA loop in a sequence-specific fashion. Second, the β2-β3 loop of the hRBMY inserts into the major groove of the RNA stem. The first binding mode might be conserved in the paralogous protein heterogeneous nuclear RNP G, whereas the second mode of binding is found only in hRBMY. This structural difference could be at the origin of the function of RBMY in spermatogenesis.
Author(s): Skrisovska L, Bourgeois CF, Stefl R, Grellscheid S-N, Kister L, Wenter P, Elliott DJ, Stevenin J, Allain FH-T
Publication type: Article
Publication status: Published
Journal: EMBO Reports
Print publication date: 01/04/2007
ISSN (print): 1469-221X
ISSN (electronic): 1469-3178
Publisher: Nature Publishing Group
PubMed id: 17318228
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