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Structural characterisation of unsaturated bacterial hopanoids by atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry

Lookup NU author(s): Dr Helen Talbot, Dr Paul Farrimond

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Abstract

The production of bacteriohopanepolyols (BHPs) is widespread in many different groups of prokaryotes; however, unsaturated components are less common except amongst the acetic acid bacteria. Here we describe the characterisation of mono- (Δ6 or Δ11) and diunsaturated (Δ6,11) bacteriohopanetetrols isolated from the acetic acid bacterium Gluconacetobacter xylinus (formerly Acetobacter aceti ssp. xylinum) by atmospheric pressure chemical ionisation ion trap mass spectrometry (APCI-MSn). APCI-MS2 spectra are compared with equivalent electron ionisation (EI) spectra and differences in fragmentation pathways are discussed. Having established characteristic spectral features for a range of unsaturated BHPs we now have the ability to rapidly detect the presence of unsaturated BHPs in both natural environmental samples (soils, sediments, water columns) as well as in microbial cultures. Copyright © 2007 John Wiley & Sons, Ltd.

Publication metadata

Author(s): Talbot HM, Rohmer M, Farrimond P

Publication type: Article

Publication status: Published

Journal: Rapid Communications in Mass Spectrometry

Year: 2007

Volume: 21

Issue: 10

Pages: 1613-1622

ISSN (print): 0951-4198

ISSN (electronic): 1097-0231

Publisher: John Wiley & Sons Ltd.

URL: http://dx.doi.org/10.1002/rcm.2997

DOI: 10.1002/rcm.2997

PubMed id: 17443490

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