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Lookup NU author(s): Professor Christine Foyer
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The aim of the present study was to provide useful background information and evidence of the functionality of the maize Activator/Dissociation (Ac/Ds) system in hexaploid wheat. Two transgenic parental wheat lines, one harbouring the immobilised Ac element (iAc) and the other the Ds element (pUbi[Ds-uidA]bar), were crossed. Transient GUS assays confirmed that the iAc transposase is active in hexaploid wheat. Selected F1 and F2 lines were analysed by PCR using primers specific to Ac, uidA and bar genes. The primer pair Ubi/bar-tag was used to detect excision of the Ds-uidA sequence, which occurred at a frequency of 39% in the F1 generation. Lines free of Ac and showing evidence of Ds excision were subject to Southern analysis, which indicated that at least one transposition event might have occurred in these lines. Although more evidence is required to unequivocally support the reintegration of the Ds element in the wheat genome, the evidence presented here nevertheless demonstrates the effectiveness and potential value of using this system to tag genes in wheat. © CSIRO 2007.
Author(s): Pastori GM, Huttly A, West J, Sparks C, Pieters A, Luna CM, Jones HD, Foyer CH
Publication type: Article
Publication status: Published
Journal: Functional Plant Biology
Year: 2007
Volume: 34
Issue: 9
Pages: 835-843
Print publication date: 01/01/2007
ISSN (print): 1445-4408
ISSN (electronic): 1445-4416
Publisher: CSIRO Publishing
URL: http://dx.doi.org/10.1071/FP07112
DOI: 10.1071/FP07112
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