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Lookup NU author(s): Dr Mauro Santibanez Koref
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Recent in silico analysis has revealed the presence of a group of proteins in pro and lower eukaryotes, but not in Man, that show extensive amino acid sequence similarity to known O6-alkylguanine-DNA alkyltransferases, but where the cysteine at the putative active site is replaced by another residue, usually tryptophan. Here we review recent work on these proteins, which we designate as alkyltransferase-like (ATL) proteins, and consider their mechanism of action and role in protecting the host organisms against the biological effects of O6-alkylating agents, and their evolution. ATL proteins from Escherichia coli (eAtl, transcribed from the ybaz open reading frame) and Schizosaccharomyces pombe (Atl1) are able to bind to a range of O6-alkylguanine residues in DNA and to reversibly inhibit the action of the human alkyltransferase (MGMT) upon these substrates. Isolated proteins were not able to remove the methyl group in O6-methylguanine-containing DNA or oligonucleotides, neither did they display glycosylase or endonuclease activity. S. pombe does not contain a functional alkyltransferase and atl1 inactivation sensitises this organism to a variety of alkylating agents, suggesting that Atl1 acts by binding to O6-alkylguanine lesions and signalling them for processing by other DNA repair pathways. Currently we cannot exclude the possibility that ATL proteins arose through independent mutation of the alkyltransferase gene in different organisms. However, analyses of the proteins from E. coli and S. pombe, are consistent with a common function. © 2007 Elsevier B.V. All rights reserved.
Author(s): Margison GP, Butt A, Pearson SJ, Wharton S, Watson AJ, Marriott A, Caetano CMPF, Hollins JJ, Rukazenkova N, Begum G, Santibanez-Koref MF
Publication type: Article
Publication status: Published
Journal: DNA Repair
Year: 2007
Volume: 6
Issue: 8
Pages: 1222-1228
ISSN (print): 1568-7864
ISSN (electronic): 1568-7856
Publisher: Elsevier BV
URL: http://dx.doi.org/10.1016/j.dnarep.2007.03.014
DOI: 10.1016/j.dnarep.2007.03.014
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