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Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulence

Lookup NU author(s): Dr Michail Karavolos, Dr David Bulmer, Dr Jeong-Jin Lee, Dr Anjam Khan

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Abstract

Pathogenic bacteria employ a variety of mechanisms to resist a barrage of stresses they encounter during active growth in or outside the host as well as during growth stasis. An in silico screen of the Salmonella genome sequence revealed that Salmonella typhimurium LT2 possesses a homologue belonging to the universal stress protein A (UspA) family. We assessed the transcriptional profile of uspA in S. typhimurium C5 by constructing a lacZ fusion revealing that uspA is induced by metabolic, oxidative, and temperature stresses. The highest transcriptional levels occurred in cells entering stationary phase, an observation consistent with expression patterns in Escherichia coli. The protein was purified as a fusion with GST (UspAF) and antibodies raised against UspAF revealed elevated protein levels in stressed and growth-arrested cells. Inactivation of uspA in S. typhimurium C5, lead to increased susceptibility to stress conditions. Furthermore, UspA makes an important contribution to the in vivo virulence of Salmonella in mice thus highlighting the importance of stress resistance regulation in pathogenicity and survival within the host. Crown Copyright © 2006.


Publication metadata

Author(s): Liu W-T, Karavolos MH, Bulmer DM, Allaoui A, Hormaeche RDCE, Lee JJ, Anjam Khan CM

Publication type: Article

Publication status: Published

Journal: Microbial Pathogenesis

Year: 2007

Volume: 42

Issue: 1

Pages: 2-10

ISSN (print): 0882-4010

ISSN (electronic): 1096-1208

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.micpath.2006.09.002

DOI: 10.1016/j.micpath.2006.09.002

PubMed id: 17081727


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